Polyamines contained by two densonucleosis viruses

Generation of biologically contained Ebola viruses.

Ebola virus (EBOV), a public health concern in Africa and a potential biological weapon, is classified as a biosafety level-4 agent because of its high mortality rate and the lack of approved vaccines and antivirals. Basic research into the mechanisms of EBOV pathogenicity and the development of effective countermeasures are restricted by the current biosafety classification of EBOVs. We theref...

Densonucleosis of bloodsucking mosquitoes

Ultrastructure, development and histopathology of a new densonucleosis vlrus of bloodsucking mosquitoes are described. Mosquito densonucleosis virus is infective to all 4 larval instars, pupae and adults of both sexes. Incubation periods were significantly shorter in older fourth-instar larvae than in third-instar larvae. Infected larvae die during the fourth larval instar or as pupae. The limi...

Densonucleosis virus structural proteins.

The protein coats of two densonucleosis viruses (types 1 and 2) were examined by a variety of biophysical, biochemical, and serological techniques. The viruses were 24 nm in diameter, contained at least four polypeptides, were remarkably stable to extremes of pH and denaturing agents, and were serologically closely related. The two viruses could, however, be distinguished serologically and by d...

Comparative Adaptation of some pox viruses in two cell systems

Metabolism of polyamines by Staphylococcus.

Rosenthal, Sanford M. (National Institute of Arthritis and Metabolic Diseases, Bethesda, Md.) and Donald T. Dubin. Metabolism of polyamines by Staphylococcus. J. Bacteriol. 84:859-863. 1962-Although spermine and spermidine are not detectable in staphylococci grown in purified medium, considerable amounts (0.7 to 1.4 mumole/g wet wt) were demonstrated in cells grown in the presence of these amin...